Cytotoxicity is a well established and easily accessable endpoint to get first information on the general / acute toxic potential of a test item. In order to further investigate the possible involvement of metabolism and the mechanism of toxicity different test systems and / or endpoints should be investigated.
- low metabolic competence: V79 cells (parental)
- high metabolic competence: primary hepatocytes (see ADME, Species Comparison and Metabolite Profiling)
- specific metabolic competence: V79 Cell BatteryTM (see V79 Cell BatteryTM)
Three different endpoints for cytotoxicity can be measured:
- mitochondrial activity: MTT-Assay
- membrane integrity: Neutral Red Uptake
- total protein content: Sulforhodamine B-Assay
Please inquire for further test systems and endpoints.
Screening Cytotoxicity / Hepatotoxicity Test
The relative cell viability upon incubation with test item compared to the solvent control is determined (single point).
Quantity of test item required: 1 mg (pre-weighted)
Turnaround time of report : 10 working days
- V79 cells, primary hepatocytes, etc.
- one concentration of the test item
- four replicates
- one incubation time
- positive control
- negative control