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GenPharmTox BioTech AG
phone: +49 (0) 89 89 55 59 - 0
eMail: info@genpharmtox.de
 
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Cytotoxicity Test

Cytotoxicity is a well established and easily accessable endpoint to get first information on the general / acute toxic potential of a test item. In order to further investigate the possible involvement of metabolism and the mechanism of toxicity different test systems and / or endpoints should be investigated.

Test-Systems

  • low metabolic competence: V79 cells (parental)
  • high metabolic competence: primary hepatocytes (see ADME, Species Comparison and Metabolite Profiling)
  • specific metabolic competence: V79 Cell BatteryTM (see V79 Cell BatteryTM)

Three different endpoints for cytotoxicity can be measured:

  • mitochondrial activity: MTT-Assay
  • membrane integrity: Neutral Red Uptake
  • total protein content: Sulforhodamine B-Assay

Please inquire for further test systems and endpoints.

Screening Cytotoxicity / Hepatotoxicity Test

The relative cell viability upon incubation with test item compared to the solvent control is determined (single point).


Quantity of test item required: 1 mg (pre-weighted)
Turnaround time of report : 10 working days


Study Design

  • V79 cells, primary hepatocytes, etc.
  • one concentration of the test item
  • four replicates
  • one incubation time
  • positive control
  • negative control


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GenPharmTox BioTech AG • phone: +49 (0) 89 89 55 59-0 • fax: +49 (0) 89 89 55 59-18 • eMail: info@genpharmtox.de

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